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Fig. S5
Effect of N-WASP-CA and Nocodazole on the number of actin patches and ADR of TfR, related to Figure 5. (A) Cortical neuron expressing EGFP and (B) TfR-mCherry, which is confined to the somatodendritic compartment. (C) Cortical neuron expressing EGFP-N-WASP-CA and (D) TfR-mCherry, which is present in the axon as well as in the somatodendritic compartment. (E) Image of a neuron in dissociated culture stained for tubulin after exposure to DMSO for 30 minutes shows intact microtubules. (F) Similar neuron to (E) exposed to Nocodazole for 30 minutes shows disruption of microtubules. (G, H) The number of patches labeled with GFP-Utr in the proximal axon of a neuron in dissociated culture in the absence and presence of Nocodazole (n = 9 neurons, 3 cultures). (I) Cortical neuron expressing GFP and (J) TfR-mCherry prior to addition of Nocodazole. (K) Same neuron as in (J) following 30 minutes of exposure to Nocodazole. (L) ADR of TfR is not statistically different in the presence (n = 10 neurons, 2 cultures) vs. absence (n = 9 neurons, 2 cultures) of Nocodazole. Inset shows Ankyrin G staining. Arrowheads point to axon. Scale bar 5 μm.