|
Fig. 2 Inhibition of Wnt/β-catenin signaling inhibits dye spreading towards the caudal transected spinal cord. Pharmacological inhibition of the Wnt/β-catenin pathway (IWR-1 treatment) reduces the proportion of animals with dextran transport beyond the spinal lesion site, indicating lack of restored spinal continuity (Fischer's exact test: ***P<0.001). In DMSO-treated control larvae, intraspinally injected fluorescent dextran is transported across the lesion site (asterisk) into the caudal spinal cord (arrow). Dextran transport is interrupted at the level of the lesion site (asterisk) in IWR-1-treated larvae. Shown is a lateral view, dorsal is up, rostral is left). Abbreviations: BF, brightfield; Rhod. Dextran, Rhodamine dextran. Scale bar: 200 µm. Error bars indicate s.e.m.