IMAGE

Fig. 1

ID
ZDB-IMAGE-180328-20
Source
Figures for Mohamed et al., 2017
Image
Figure Caption

Fig. 1

Expression of GtACR1 and GtACR2 in transgenic zebrafish. a, b Expression of GtACR1-eYFP in the trunk of 1-day-old GAL4s1020t, UAS:GtACR1-eYFP (a) and GAL4s1020t, UAS:GtACR2-eYFP (b) embryos. Labelled cells are located in the ventral regions of the spinal cord. c, d High magnification view of the trunk of fish expressing GtACR1-eYFP (c) or GtACR2-eYFP (d) in spinal neurons. There is label in the plasma membrane (arrows). For GtACR2, the label is dimmer. Puncta are visible (arrowheads). e An 8-dpf gng8:GAL4, UAS:GtACR1-eYFP larva, showing label in habenula neurons and in axons innervating the interpeduncular nucleus. f Expression of GtACR1-eYFP in olfactory neurons of a 3-day-old fish carrying the GAL4s1011t driver. g, h Forty-eight-hour-old embryos labelled with acridine orange. Dying cells are strongly labelled and appear as bright spots. These are detected in both GtACR1-expressing (g) and non-expressing (h) fish. There is no evidence of cell death in the ventral spinal cord of GtACR1-expressing fish. Anterior is to the left in all panels except f, where anterior is to the top. All panels are lateral views, except e and f, which are dorsal views. Panels a, c, d and f are single planes, while b, e, g and h are maximum projections. OE olfactory epithelium, IPN interpeduncular nucleus, lHb left habenula, rHb right habenula

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.