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Fig. 7
Zebrafish as a model to study fecal shedding and transmission. (A) Scheme depicting experimental setup of shedding and transmission experiment. Infected AB embryos were transferred into fresh media housing naive Tg(mpo::egfp) embryos (both 4 dpf). Posttransfer, shedding of EHEC into the media and transfer to recipient animals were monitored over time. (B) EHEC shed into the media were enumerated immediately and at 6, 12, and 24 h posttransfer of infected AB embryos into fresh media. Shown are individual data points, means, and SD (n = 5/time point). (C) The bacterial burden of Tg(mpo:egfp) recipient embryos was quantified immediately and at 6, 12, and 24 h posttransfer of infected AB (donor) embryos. Shown are individual data points, means, and SD (n = 10 embryos/time point). Statistical significance was calculated using the Kruskal-Wallis test with Dunn’s multiple-comparison test (*, P < 0.05). (D) Example of EHEC::mCherry localization in recipient embryos.