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Fig. 6
The zPDX can be efficiently established using human CRC primary samples. Cell suspensions derived from surgically resected human colon tumors were labeled with the lipophilic DiI dye (red) and injected into the PVS of 48-hpf wt or Tg(fli:EGFP). At 4 dpi, the number of zebrafish with an implanted tumor was quantified (A–F) (each dot represents the implantation percentage of each experiment). Representative confocal images of 4-dpi zPDX showing neovasculature in Tg(fli:EGFP) (A′–E′) and tumor masses with high cytomorphologic and architectural diversity (DAPI) (A′′–E′′). The number of nuclei (tumor size) (G) and mitotic figures (H) in these tumor masses was quantified; each dot represents one xenograft. Representative images of mitotic figures (A′′′–E′′′) (red arrows) and corresponding quantification (H). HLA and human mitochondria-immunostained cells at 24 hpi (I) and 4 dpi (J). Tubular structures with luminal CEA staining (K and L). (Scale bar, 50 μm.) *P < 0.05; **P < 0.005; ***P < 0.0001; ns, nonsignificant.