ZFIN Image: Wakisaka et al., 2017, Fig. S5

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Figure Caption

Fig. S5 TNAP is expressed in non-neuronal cells predominantly located in the anterior OE close to the inlet naris. Related to Figure 6.
(A) Double fluorescence in situ hybridization with cRNA probes for TNAP (green) and OMPs, TRPC2s, or V1Rs (magenta) on OE sections. No overlap is observed for TNAP signals with any OSN markers, indicating that TNAP is expressed in non-neuronal cells in the OE (n=3). Scale bars, 20 μm.
(B) Distribution of TNAP- and A2c-expressing cells in the zebrafish OE. Top left, a schematic diagram showing water flow through the zebrafish OE. Top right, a representative in situ hybridization image of TNAP expression in an OE section. Each OE section was divided into 10 compartments along the longitudinal axis and the numbers of TNAP and A2c-positive cells were counted. Bottom, a graph showing distribution of TNAP (red)- and A2c (blue)-expressing cells in the OE (n=3). Note that TNAP-expressing cells are predominantly located in the vicinity of the OE inlet, whereas A2c-expressing OSNs are observed in the middle portion between the inlet and outlet.

Acknowledgments

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