ZFIN Image: Favre-Bulle et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Optical setup for otolith OT and behavioural imaging. Dorsal (a) and lateral (b) views, indicating the location of the utricular otoliths (circles) in a 6dpf zebrafish larva (scale bars, 200 µm). c Experimental set up (see Supplementary Methods for details) for delivering a dual OT to the larva using a 1064 nm fibre laser, a half-wave plate (HWP), polarising beam splitters (PBS), gimbal-mounted mirrors (GM), a dichroic mirror (DM), and lenses to project the two traps into the sample via a 20X 1NA microscope objective. Camera 1 (d) allows targeting of the OTs and imaging of the eyes (scale bar, 200 µm), and camera 2 (e) permits imaging of tail movements (scale bar, 600 µm)

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