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Figure Caption
Fig. 3 Locating spinal motor nerves in adult zebrafish transverse sections. A: Immunohistochemistry on floating sections using an antibody to tubulin. Yellow arrowheads identify the tubulin+ peripheral motor nerves. Scale bar = 100 µm. B: Black Gold II myelin stain to label the myelin sheath. The dotted box denotes the area enlarged in the right and bottom right images. White arrowheads identify the myelin sheath associated with peripheral motor axons. C: Osmium labeling identifies the myelin sheath associated with peripheral motor axons (white arrowheads).
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