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Fig. 3
Midazolam or ketamine pretreatment decreases the neuronal apoptosis after cardiac arrest. a-d TUNEL staining of control (a), CA (b), CA + midazolam (0.5 mM)(c) and CA + ketamine (2.5 mM) pretreatment (d) zebrafish at 7 dpf. Red, TUNEL staining, indicating the apoptotic cell; Blue, DAPI-stained nuclei. Scale bar: 10 μm. e Quantification of percentages of TUNEL positive cells in each group (mean ± s.e.m.; *** P<0.001, one-way ANOVA plus Newman–Keuls multiple comparison test). Numbers in the histograms represent the number of sections analyzed in each group
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