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Fig. 4
Bone development of offspring was impaired through maternal transmission of topiramate. (A–F) Ossification status of cranium and vertebral regions was detected by Alizarin red staining of zebrafish at 10 dpf; (G) Vertebrae numbers from offspring of control or topiramate-treated female zebrafish (♀25) (averages ± SD; *** p < 0.005; control n = 15; topiramate-treated n = 82). (ch, ceratohyal; cl, cleithrum; m, Meckel’s cartilage; Scale bar: 100 μm).
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Full text @ Int. J. Mol. Sci.