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Fig. S2
Expression of Cldn5 in KV was ablated by two types of cldn5a translation-blocking MO.
(A—F) Maximum intensity projection images of Cldn5 (red) and sox17:egfp-positive KV cells (green) in 6 ss embryos. Representative images of standard control MO injected embryo (n = 9) (A), cldn5a translation-blocking MO1 injected embryo (n = 11) (B), cldn5a translation-blocking MO2 injected embryo (n = 11) (C), DFC-specific control morphants (n = 8) (D), DFC-specific cldn5a MO1 injected embryo (n = 6) (E), and DFC-specific cldn5a MO2 injected embryo (n = 7) (F). Scale bar: 20 μm.
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