Fig. S2

Fig. S2 Heart looping and downregulated expression of spaw and its downstream genes were not caused by induction of p53 expression in klf8 morphants. Embryos co-injected with p53-MO^sp and klf8-MO1^atg or klf8-MO2^atg displayed no-loop or L-loop heart defects at 72 hpf (A). The majority of embryos co-injected with p53-MO^sp and klf8-MO1^atg or klf8-MO2^atg exhibited decreased or absent expression of spaw (B), lft1 (C), lft2 (D), or pitx2 (E) in the left LPM, diencephalon or heart at the 18–22 s stages. Expression levels of gata5 and oep which are known to be expressed in the LPM at the 22 s stage were unaffected by klf8 knockdown (F-M).