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Fig. S3
Mutations in hmgcs1 do not affect sonic hedgehog signaling.
A-B. Whole mount in situ hybridization was performed on wildtype and mutant siblings at 3 days post fertilization/protruding mouth stage to detect the expression of ptch2. n = 47 including 17 wildtype, 7 homozygous mutants, and 23 heterozygous mutants. C. Real time PCR was performed on a pool (n = 20) of siblings or hmgcs1 mutant embryos at 4 days post fertilization to detect ptch2 expression (ptc2). Error bars demonstrate the standard error of the mean across two biological replicates each with 20 embryos per pool.
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