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Fig. 5
Multicolor two-photon efficient imaging of endogenous fluorophores in zebrafish embryo Images of the blue, green, red fluorescence, second harmonic generation, and merged channels of a live zebrafish embryo recorded 48 hours post fertilization (a) Large-scale (stitched mosaic) image encompassing 2450 × 730 µm2. (b) Detail extracted from the large-scale image. Pixel size, 0.83 µm. Multicolor pixel acquisition time, 40 µs. Scale bar, 500 µm.
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