ZFIN Image: Wu et al., 2017, Fig. S3

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Figure Caption

Fig. S3

Targeting specificity of cpn1 ATG morpholinos. (A) At 24hpf, embryos injected with mRNA from the cpn1-atg-GFP construct results in GFP expression (n=24/25 with GFP). (B) Co-injection of (cpn1-atg-GFP) mRNA and cpn1 MO reduced and/or blocked the green signal in the embryos (n=2/22 with GFP). The images are the representative pictures from two independent experiments. (C) Western blot analysis showed the reduction of Cpn1 protein levels significantly by morpholino knockdown of cpn1 to block translation or to interfere with cpn1 splicing. Rabbit polyclonal anti-Cpn1 antibody (TA340008) is purchased from OriGene and β-actin serves as a loading control. The data shown is representative of two independent experiments.

Acknowledgments

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