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Fig. 2
Regeneration of the zebrafish caudal fin and time courses of total regenerated area (TRA), vascular projection area (VPA), vessel wall (contour) length (CL) and their derivatives during regeneration.
The process of caudal fin regeneration at multiple time points from reference to 50 dpa: the upper row (A) represents bright field images at reference, 1, 3, 5, and 7 dpa. The second row (B) contains fluorescent images at reference, 7, 14, 28, and 50 dpa. Such images allowed quantitative assessment of time courses of multiple general and vascular parameters as presented in the graphs C-C'', D and D'. At least 50% of the cut fin (TRA) is regenerated within the first 7 days (C) and the rapid angiogenesis between day 3 and 7 leads to increase of the vessel area values almost up to the reference level (C'). The levels of contour length reached reference level at 10–14 dpa. Vessel area density (D) as well as average vessel diameter (D') were remarkably higher in comparison with the reference up to 7 dpa.