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Fig. 4
VPA and TSA delay the development of exocrine pancreas.
The development of exocrine pancreas was analyzed at 72 hpf, 96 hpf and 120 hpf. GFP fluorescence in embryos of the ElastaseA-GFP transgenic line: controls (A-D), embryos treated with 0.2 μM TSA (D-F). White arrows indicate the exocrine pancreas. Whole-mount in situ hybridizations to detect trypsin transcripts (G-X) in differentiated pancreatic exocrine cells of control embryos (G-I), embryos treated with increasing concentrations of TSA (J-R) or VPA (S-X). Black arrows indicate the exocrine pancreas.
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