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Fig. 3
HiLo microscopy enhances calcium signals.
(A) In vivo HiLo image of motor neurons of a zebrafish embryo expressing GCaMP5G in soma (ROI1), axons (ROIs 2–4), or background (ROIs 5–7). Scale bar 5 μm. (B) ΔF/F time series in the soma (ROI 1) shows that HiLo mode leads to a larger signal than UWF mode. (C) For axons (ROI 4) the gain in HiLo mode is even larger. Acquisition rate was 25fps.
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