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Fig. 4
Reduction of tyrosine hydroxylase (TH)+ neurons in PINK1-deficient (PINK1-KO) zebrafish.
(A) Sequence information of PINK1-deficient zebrafish (c.176_177del, PINK1Δ2/Δ2). The red letter indicates the target sequence of the guide RNA, and the red square indicates the site of the 2-bp deletion. (B) Representative images of dopaminergic neurons in the posterior tuberculum and noradrenergic neurons in the locus coeruleus. Right panel: magnified pictures of the red square regions. (C) The numbers of dopaminergic neurons in the posterior tuberculum and noradrenergic neurons in the locus coeruleus were significantly decreased in PINK1-deficient zebrafish (PINK1Δ2/Δ2) (4-month-old zebrafish); n = 5 fish per group. Bars represent the SEM. *, p < 0.05, **, p < 0.01.