PUBLICATION
An optimized method for counting dopaminergic neurons in zebrafish
- Authors
- Matsui, H., Sugie, A.
- ID
- ZDB-PUB-170908-2
- Date
- 2017
- Source
- PLoS One 12: e0184363 (Journal)
- Registered Authors
- Matsui, Hideaki
- Keywords
- Neurons, Dopaminergics, Zebrafish, Locus coeruleus, Parkinson disease, Fish, Mammals, Tyrosine
- MeSH Terms
-
- Zebrafish
- Animals
- Dopaminergic Neurons/cytology*
- Dopaminergic Neurons/drug effects
- Dopaminergic Neurons/metabolism*
- Oxidopamine/pharmacology
- Disease Models, Animal
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism
- Parkinson Disease/genetics
- Parkinson Disease/metabolism
- PubMed
- 28880915 Full text @ PLoS One
Citation
Matsui, H., Sugie, A. (2017) An optimized method for counting dopaminergic neurons in zebrafish. PLoS One. 12:e0184363.
Abstract
In recent years, considerable effort has been devoted to the development of a fish model for Parkinson's disease (PD) to examine the pathological mechanisms of neurodegeneration. To effectively evaluate PD pathology, the ability to accurately and reliably count dopaminergic neurons is important. However, there is currently no such standardized method. Due to the relatively small number of dopaminergic neurons in fish, stereological estimation would not be suitable. In addition, serial sectioning requires proficiency to not lose any sections, and it permits double counting due to the large size of some of the dopaminergic neurons. In this study, we report an optimized protocol for staining dopaminergic neurons in zebrafish and provide a reliable counting method. Finally, using our optimized protocol, we confirmed that administration of 6-hydroxydopamine (a neurotoxin) or the deletion of the PINK1 gene (one of the causative genes of familiar PD) in zebrafish caused significant reduction in the number of dopaminergic and noradrenergic neurons. In summary, this method will serve as an important tool for the appropriate evaluation and establishment of fish PD models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping