IMAGE

Fig. 3

ID
ZDB-IMAGE-170921-55
Source
Figures for Sedykh et al., 2017
Image
Figure Caption

Fig. 3

Pax2a expression is aberrant in MZ-zic2 mutants at 1 dpf. pax2a expression at 1 dpf was visualized in embryos derived from zic2agbt133/+; zic2buw1116/+ parents using WISH (A-F) or in progeny of zic2agbt133/+; zic2buwt104 parents using immunohistochemistry (G-L). A: normal pax2a expression in the ventral retina (*). B: mispatterned pax2a expression (*) was observed in 12 out of 103 embryos (12%, 2 expts.). C: Only zic2b homozygous embryos exhibit pax2a mispatterning. zic2a genotype was not tested because PCR genotyping was not robust after WISH. D-F: Embryos with mispatterned pax2a expression also exhibit coloboma, indicative of homozygosity for zic2agbt133. G, H: confocal stacks through representative retina of normal (G) and zic2 mutant (H) retina. I, J: confocal stacks through the ventral aspects of a normal (I) and zic2 mutant (J) diencephalon and retina. Arrowheads in H, J point to the aberrant optic stalk. In G-J, yellow = Pax2a, magenta = F-actin cytoskeleton visualized by phalloidin. K, L: single confocal sections through representative normal (K) and zic2 mutant (L) embryos, imaged ventrally at the level of choroid fissure. magenta = Pax2a; yellow = acetylated tubulin; cyan = nuclei visualized by DAPI. Embryos are shown in lateral views, anterior to the left (A-F) or anterior to the right (G, H); in ventral views with anterior at the top (I-L).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 429(1), Sedykh, I., Yoon, B., Roberson, L., Moskvin, O., Dewey, C.N., Grinblat, Y., Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis, 92-104, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.