IMAGE

Fig. 1

ID
ZDB-IMAGE-170825-31
Genes
Antibodies
Source
Figures for Wehner et al., 2017
Image
Figure Caption

Fig. 1

Regenerating axons navigate a non-neural lesion environment that is rich in ECM independently of astroglia-like processes. a Time-lapse video-microscopy reveals that axonal growth cones (arrow, Xla.Tubb:DsRED) extend into the lesion site independently of astroglia-like processes (her4.3:EGFP, empty arrow points out lack of glial labelling). Arrowhead indicates glial process extending into the lesion site. Single frames are shown. Abbreviations: fin, dorsal fin fold; sc, spinal cord; nc, notochord. b Quantification of fascicle composition at 1 dpl using different combinations of immunohistochemical and transgenic markers for astroglia-like processes and axons indicates that the majority of fascicles are neuronal with no detectable glial component (46–62% of all fascicles analyzed). In the example scans on the left, yellow arrows indicate mixed fascicles, containing neurites and glial processes; green arrows indicate pure glial fascicles and red arrows indicate pure axonal fascicles. c Time course of re-growth of axons (anti-acetylated Tubulin+) and astroglia-like processes (anti-GFAP+) after spinal cord transection. Quantification of labelling continuity between rostral and caudal spinal cord stumps at 1 dpl and 2 dpl suggests faster bridging of the lesion site by neuronal rather than glial processes. Arrow points to axonal bridge with very few glial processes in the lesion site at 1 dpl at higher magnification (Fischer’s exact test: **P < 0.01; n.s. indicates not significant). d Treatment of gfap:Gal4ff;UAS:NTR-mCherry transgenic fish with the pro-drug Metronidozole (MTZ) almost completely ablates transgene-expressing glial cells by 3 dpf. e Treatment of gfap:Gal4ff;UAS:NTR-mCherry transgenic fish with MTZ dramatically reduces anti-GFAP+astroglia-like processes (t-test: ***P < 0.001) but does not reduce the proportion of larvae with axonal bridges at 2 dpl (Fischer’s exact test: n.s indicates not significant). f fn1a and col1a2 expression is upregulated after lesion, shown by in situ hybridization. g Regenerating axons (anti-acetylated Tubulin+; arrows) are closely associated with Collagen I and Fibronectin immunoreactivity in a lesion site (confocal depth was limited to spinal cord). Also see Supplementary Movies 3 and 4. ag Views are dorsal (a; rostral is left) or lateral (b-g; dorsal is up, rostral is left). BF: brightfield. Scale bars: 100 µm a,cf and 50 µm b,g. Error bars indicate s.e.m

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.