IMAGE

Fig. 5

ID
ZDB-IMAGE-170620-13
Source
Figures for Erickson et al., 2017
Image
Figure Caption

Fig. 5

Tomt-deficient hair cells do not label with MET-channel permeant FM dyes.

(A–D) FM 1–43 dye label of lateral line hair cells in 2 dpf (A, B) and 6 dpf (C, D) wild-type siblings and tomttk256c mutants. (A, C) Representative DIC images of NM hair bundles (left), fluorescence images of FM 1–43 in the same NMs (middle), and a merge of the FM 1–43 images with DIC images of the hair cell bodies (right) from 2 dpf (A) and 6 dpf (C) wild-type siblings and tomttk256c mutants. (B, D) Quantification of FM 1–43 fluorescence intensity per NM of 2 dpf (B) and 6 dpf (D) wild-type siblings (n = 6 larvae; 2 NMs each) and tomttk256c mutants (n = 8 larvae; 2 NMs each). Error bars are the mean ± SD. Asterisks indicate p<0.0001 by unpaired, two-tailed t-test. (E) Quantification of hair cell number per neuromast in 2 dpf and 6 dpf tomttk256c mutants and wild-type siblings (same as those shown in panels A – D). The box plots cover the 25th to 75th percentiles, and the whiskers represent the minimum and maximum counts. ns = not significant, asterisks indicate p<0.0001 by unpaired, two-tailed t-test. (F) Representative images of FM 1–43 labeling of inner ear hair cells in 6 dpf wild-type siblings (n = 5 larvae) and tomtnl16 mutants (n = 7 larvae). (G) Rescue of FM dye labeling in mercury mutants by stably expressed Tomt-GFP. Representative images of Tomt-GFP (left panels) and FM 4–64 (right panels) in lateral line NMs of a Tg(myo6b:tomt-GFP) wild-type sibling and a Tg(myo6b:tomt-GFP);tomttk256c mutants at 5 dpf. Tomt-GFP and FM 4–64 images are from the same NM for each genotype. (H) Quantification of FM 4–64 fluorescence intensity per NM for 5 dpf non-transgenic wild-type siblings (n = 6 larvae, 8 NMs), non-transgenic tomttk256c mutants (n = 6 larvae, 12 NMs), Tg(myo6b:tomt-GFP) wild-type siblings (n = 7 larvae, 14 NMs), and Tg(myo6b:tomt-GFP);tomttk256c mutants (n = 6 larvae, 12 NMs), including those NMs shown in F. Error bars are the mean ± SD. ns = not significant. Asterisks indicate p<0.0001 by one-way ANOVA with Bonferroni's multiple comparison test. Scale bars: 5 µm in A, C, F, and G.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife