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Fig. 1

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ZDB-IMAGE-170530-6
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Figures for Wan et al., 2017
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Figure Caption

Fig. 1

fgf8a Gene Regulation during Retina Regeneration

(A) RT-PCR analysis of fgf8a, fgf8b, and Fgf-responsive genes in uninjured and needle-poke-injured retinas.

(B) In situ hybridization assays and BrdU immunofluorescence for fgf8a expression and MG proliferation, respectively, before and after injury to central retina in 6-month-old fish. Arrowheads point to fgf8a RNA in uninjured retina; arrows point to fgf8a RNA enriched in proliferating MG-derived progenitors at 4 dpi. Asterisk indicates injury site. Scale bar, 50 μm.

(C) RT-PCR analysis of ascl1, fgf8a, and gapdh RNAs in GFP+ MGs and GFP− non-MGs (retinal neurons) in uninjured and injured retinas that were FACS purified from gfap:GFP transgenic fish retinas.

See also Figure S1.

Acknowledgments
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