IMAGE

Fig. 5

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ZDB-IMAGE-170413-57

Genes

Source

Figures for Carroll et al., 2014

Image

Figure Caption

Fig. 5

Xenoestogens Partially Replicate the Effects of E2 on HSPC Formation

(A) Exposure to xenoestrogens ethinyl estradiol (EE), genistein (GEN), or Bisphenol A (BPA) decreased runx1/cmyb expression and could be partially blocked by cotreatment with FULV (n ≥ 25/treatment).

(B) Treatment with GEN reduced the expression of fli1/flk1 in the intersomitic vessels (n ≥ 25/treatment); an arrow points to ISVs.

(D) VEGFAa was noticeably decreased by EE (15/26); arrowheads indicate somite region.

(E) Summary of changes in the expression of markers of vascular identity ephrinB2, flt4, notch3, and deltaC following treatment with XEs (n ≥ 25).

(F) qPCR confirmed decreases in runx1, cmyb notch3, and scl by XE exposure (mean of triplicate experiments ± SEM; one-tailed t test ^∗p < 0.05; ^∗∗p < 0.01).

Figure Data

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Acknowledgments

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Reprinted from Developmental Cell, 29, Carroll, K.J., Esain, V., Garnaas, M.K., Cortes, M., Dovey, M.C., Nissim, S., Frechette, G.M., Liu, S.Y., Kwan, W., Cutting, C.C., Harris, J.M., Gorelick, D.A., Halpern, M.E., Lawson, N.D., Goessling, W., North, T.E., Estrogen defines the dorsal-ventral limit of VEGF regulation to specify the location of the hemogenic endothelial niche, 437-53, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell