ZFIN Image: Bielczyk-Maczyńska et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Loss-of-function nol9 mutation leads to a defect in ITS2 pre-rRNA processing.

(A) Expression pattern of nol9 by WISH at sphere stage (4 hpf), 12 hpf, 48 hpf, 72 hpf, 96 hpf and 120 hpf. Black arrows indicate branchial arches, white arrow indicates pancreas and arrowhead indicates nol9-expressing cells in the CHT. (B) Representative Northern blot analysis of RNA isolated from 5 dpf nol9^{sa102/sa10222} mutants and control (nol9^+/+ and nol9^+/sa1022) siblings using 5’ETS, ITS1 and ITS2 probes to detect rRNA processing intermediates. Corresponding rRNA intermediates (a, b, c, d) are indicated in B) and C). (C) Schematic representation of the rRNA intermediates detected in the Northern blot analysis. The sites of hybridization of the 5’ETS, ITS1 and ITS2 probes are indicated in red. (D) Methylene blue staining of the membrane was used to control for equal loading of RNA. wt–nol9^+/+/nol9^+/sa1022, mut–nol9^{sa1022/sa1022}.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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