Fig. S1

Fig. S1

Phenotypes of ttn.2 and ttn.1 mutants. A, Cardiac edema with decreased ventricle size (dashed circles) was noted in both ttn.2^xu064 and ttn.2^xu065, but not in ttn.1^xu066 and ttn.1^xu067. Shown are bright-field images at 2 dpf. Scale bar, 0.05 mm. B and C, In ttn.2^xu064 and ttn.2^xu065, the heart rate was slightly decreased (B), and the ventricular shortening fraction was reduced to zero (C). * and ** indicates p <0.05 and p <0.01, respectively . Means ±S.D. N=5. D, Bright-field images demonstrated that the cardiac edema was still present in both ttn.2^xu064 and ttn.2^xu065 at 3 dpf, but muscle disarray was more obvious in ttn.2^xu065. Both ttn.2 mutants had whole-body edema at 7 dpf. ttn.1^xu066 and ttn.1^xu067 showed deflation of the swim bladder at 5 dpf (arrows). Scale bar, 1 mm. E, ttn.2^xu064 and ttn.2^xu065 mutants started to die at 7 dpf and could survive up to 10 dpf; ttn.1^xu066 could survive until 12 dpf; and ttn.1^xu067 started to die at 11 dpf and could live up to 17 dpf.