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Fig. S4

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ZDB-IMAGE-161104-43
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Figures for Schulte et al., 2014
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Fig. S4

In vivo complementation of MEIS1, using a previously reported MO (meis1_MO2) against the acceptor splice junction of exon 2. (A) The effect of meis1_MO1 targeting the donor site of exon 2 and inducing skipping of exons 2-7 has been evaluated previously5. (B) We here amplified the cDNA sequence between exons 2-6 in control embryos and morphants injected with 9ng meis1_MO2 and detect only ~30% of the wild-type RNA message in the morphant embryos. (C-F) Suppression of meis1 results in a reduction of the size of the optic tectum by 20-30%, similar to the phenotype observed when using meis1_MO1 that can be significantly and reproducibly rescued upon co-injection with wt human MEIS1 mRNA. Asterisks denote significance levels as determined by Student`s t-test (** p-value < 0.005; * p-value < 0.05). MO=morpholino, wt=wildtype

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