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Fig. 4
Morphants of marco have suppressed induction of pro-inflammatory cytokine response to M. marinum. (A-C) Effect of marco knockdown on the response of il1b, il8 and mmp9 to M. marinum infection. Expression levels of (A) il1b, (B) il8 and (C) mmp9 were determined by qPCR for marco morphants and their control embryos at 4 hpi after infection with M. marinum (200 cfu) and mock PVP injected controls; (D) Effect of marco knockdown on il1b expression in time (2-6 hpi). Expression was compared against the control PVP sample of each time point. All graphs show data combined from three biological replicates (n = 15 per group, pooled per replicate).