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Fig. 4

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ZDB-IMAGE-160926-3
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Figures for Thambyrajah et al., 2016
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Fig. 4

Gfi1aa expression in haemogenic endothelial cells is induced downstream of VegfA and Notch signaling, but independent of Runx1. Fixed qmc551;gata1:dsRed double transgenic embryos after GFP/dsRed immunohistochemistry are shown in (A, D-E, I). Fixed wt, qmc551 and qmc551;gata1:dsRed double transgenic embryos stained by WISH are shown in (B-C, H, K), (L) and (J), respectively. Live qmc551 embryos that were wt, heterozygous or homozygous mib carriers were imaged in (F-G). Confocal images of optical sagittal sections through the DA are 1.6 and 0.995 µm (A), 6.5 and 6.6 µm (D), 1.2 µm (E) and 2.7 µm (I) thick. A confocal maximum intensity projection of a 37 µm optical slice is shown in (G). Embryos were treated with DMSO, the VegfR inhibitors 676,475 (A) and SU5416 (B,C) or DAPM (D) from tailbud stage (10 hpf). Rbpja/b (E) and runx1 (I-L) morpholinos were injected at 2–4 cell stage. PrRBCs, HECs and inner ear hair cells are labeled with red, green and yellow arrows, respectively. Arrowheads mark reduced or absent staining. Fractions x/y give the number of embryos, x, with depicted phenotype out of all embryos analyzed, y. Embryos are shown with anterior left and dorsal up.

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Reprinted from Developmental Biology, 417(1), Thambyrajah, R., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R.N., McMahon, K., Moore, C., Gering, M., A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis, 25-39, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.