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Fig. S5
No effect on serotonin content in hypothalamus or on zebrafish morphology of expression of dominant-negative αi G-protein signaling peptide. (A) DRN DNGαi expressing larvae do not have significantly different serotonin content in the hypothalamus (normalized serotonin stain: control = 1.004 ± 0.063; DN-Gαi = 0.996 ± 0.080, P = 0.14, 2-sided student’s T-test). (B) Representative image of tg(Tph2:Gal4ff;UAS:DNGai) (DN-Gαi) or control 6 dpf transgenic zebrafish larva. Scale bars 500 µm.
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