ZFIN Image: Ye et al., 2016, Fig. S14

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Figure Caption

Fig. S14

Transplanted endoderm-committed blastomeres (ECBs) can differentiate into pancreatic endocrine cells in β cell ablated host larvae. (A-C′) Merged and single channel confocal planes of chimeras composed of Tg(sox17:GFP) ECBs transplanted into β cell-ablated host larvae, shown at 2 days post transplantation (dpt)/6 dpf. Chimeras were immunostained for GFP (green), Insulin (red), Glucagon (white), and DAPI (blue). (A,B) Control donor ECBs, and (C) dnIRS2-GFP mRNA-injected ECBs. With insulin signaling blockade, insulin+ β cell generation was increased in donor ECBs, while Glucagon+ α cell generation was decreased.

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.