ZFIN Image: Chiu et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Loss of MMP and caspase activation by BPIQ. a H1299 cells were exposed to media containing the indicated concentrations of BPIQ or vehicle control for 24 h, stained with DiOC₂(3), then analyzed for changes in their fluorescent profile by flow cytometry. b Quantitative analysis. Data are presented as means ± S.D. Histograms represent one of three independent experiments. *p < 0.05 and **p < 0.001 against vehicle control, respectively. c Western blot analysis demonstrating BPIQ-induced cytochrome c release and cleavage of caspase-9 and 3, as well as PARP. β-actin was measured as an internal control. d The distribution of cytochrome c in the cytosol of two NSCLC cell lines A549 and H1299 following 2 µM BPIQ treatment. ª mitochondria; ª cytochrome c; ª DAPI; ª co-localization of mitochondria and cytochrome c. Magnification 200 x

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