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Fig. 7
Non-ciliary function of Foxj1a regulates charon expression. (A-F) Overexpression of DBD-Foxj1a inhibits Foxj1a target gene expression. Injection of 2ng of DBD-Foxj1a mRNA suppressed dnah9 (B, 8/14) and efhc1 (E, 6/14) transcript levels, and injection of 10ng of mRNA nearly abolished dnah9 (C, 18/21) and efhc1 (F, 13/15) expression. Shown are dorsal views of 90% to 95% epiboly staged embryos. (G-I) Injection of DBD-Foxj1a mRNA did not affect charon expression at doses of either 2ng (H) or 10ng (I). Shown are dorsal views of tailbud region at 10 to 12 somites. (J-L) Overexpression of DBD-Foxj1a results in defective ciliogenesis. Shorter (K,L) and fewer (L) KV cilia were visualized at the 10-somite stage by anti-acetylated α-tubulin staining. Scale bar: 20µm. (M,N) Quantification of cilia length and number. 9 to 11 embryos were analyzed for each group. Data are represented as mean±s.d. *P<0.01 compared with controls. (O) Overexpression of DBD-Foxj1 randomizes left-sided spaw expression. Percentage of embryos with specific patterns of spaw expression was scored at the 21-somite stage. DBD, DNA-binding domain; A, absence; B, bilateral; L, left side; R, right side.