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Fig. 6
Foxj1a confers Wnt-regulated charon expression in KV. (A,B) Foxj1a is necessary for charon expression. Injection of foxj1a MO (2ng) into 1-cell staged embryos led to reduction of charon levels (B, 18/20). (C-H) Foxj1a overexpression rescues charon levels in embryos with reduced Wnt signaling. charon transcript levels were downregulated by DFC-targeted injection of fzd10 MO (4ng) (C, 28/29) and by Dkk1 induction (F, 26/30), which were restored by injection of foxj1a mRNA (2ng) (D, 23/28; G, 25/32). Injection of foxj1a mRNA alone did not significantly affect charon expression (H, 16/16). Tg(hsp:dkk1-GFP) embryos were heat shocked at 70% epiboly, and GFP+ (Dkk1+) embryos were selected under fluorescence microscope. Shown are dorsal views of tailbud region at 10 to 12 somites.