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Fig. 5
Foxj1a mediates CALef1-induced ectopic cilia formation, not tissue formation. (A-E) Injection of CAlef1 mRNA (100pg) into a single cell at the 128-cell stage resulted in formation of an ectopic protrusion (A), formation of ectopic cilia and ZO-1-positive cells (B), and ectopic expression of sox17 (C), charon (D), and ntla (E). (F-J) Coinjection of foxj1a MO (150pg) with CAlef1 mRNA (100pg) into a single cell at the 128-cell stage shortened ectopic cilia length (G) and abolished ectopic charon expression (I), but had no effect in the formation of an ectopic protrusion (F) or ectopic expression of ZO-1 (G), sox17 (H), and ntla (J). Shown are embryo morphology at the 16- to 20-somite stage (A and F), in situ staining at 10 somites (C-E,H-J), and JB-4 sections of the protrusions after whole-mount double immunohistochemical labeling using antibodies against ZO-1 (red) and acetylated α-tubulin (green) (B and G). The arrow indicates ectopic protrusions (A, F) and ectopic gene expression (C-E,H,J). Scale bars: 20µm.