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Fig. S6 Effects of Foxj1a overexpression. Injection of foxj1a mRNA (100 pg) into a single cell at the 128-cell stage did not induce ectopic expression of sox17 (B) and charon (D). gfp RNA (100 pg) was coinjected to label cells overexpressing Foxj1a (A and C). Shown are lateral views of 10-somite staged embryos. Individual embryos were photographed for GFP fluorescent signals first, and then subjected to in situ hybridization. Signals of GFP fluorescence and in situ staining from the same embryos are shown in parallel.
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