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Fig. 1
The effect of Wnt activation on foxj1a expression and cilia formation. (A-D) Wnt3a induction transiently enhances foxj1a levels in DFCs. Tg(hsp:wnt3a-GFP) embryos were heat activated at 30% epiboly for 30min, and GFP+ (Wnt3a+) embryos were selected under fluorescence microscope. foxj1a transcript levels were examined at 1h (A,B) and 4h (C,D) after heat shock. Shown are dorsal views of embryos at 50% epiboly (A,B) and 95% epiboly (C,D). (E-H) Targeted overexpression of Wnt8a results in ectopic foxj1a expression and ectopic cilia formation. Schematic diagram shows a single cell injection at the 128-cell stage (E). Injection of wnt8a mRNA (100pg) into a single cell at the 128-cell stage induced an ectopic protrusion (arrow in F), ectopic foxj1a expression (arrow in G, 8/8), and ectopic cilia-like structures as manifested by immunostaining using an antibody against acetylated α-tubulin (H). Whole-mount immunostaining was performed first, and then the protrusion was removed and imaged. Scale bar: 20µm.