Image
Figure Caption

Fig. S1 Expression of the zebrafish V-ATPase accessory protein Atp6ap1b. (A) RT-PCR of temporal expression of atp6ap1b and atp6ap1a through the first three days of development. β-actin was used as a loading control. (B) Schematic diagram and alignment of human, mouse and zebrafish Atp6ap1 proteins. (C) RT-PCR detected maternal mRNA (at the 1-cell stage) of several V-ATPase subunits and then sustained expression through 2dpf. β-actin mRNA was used as a loading control. (D) Homozygous atp6ap1ba82/a82 mutants developed pigmentation phenotypes relative to wild-type siblings. (E) Western blot analysis of Atp6ap1 in wild-type and atp6ap1ba82/a82 mutant embryos at 3, 5 and 7 dpf. A prominent band just under 50 kDa was reduced in mutants. Tubulin was used as a loading control. The graph depicts relative intensities of the Atp6ap1 band from one representative experiment. (F) Fluorescent immunostaining of Atp6ap1 in surface cells of wild-type and atp6ap1ba82/a82 mutant embryos at 3, 5 and 7 dpf. The graph shows the average fluorescent intensity of Atp6ap1 staining.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.