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Fig. 2
Changes to jaw joint morphology between 3 and 5 dpf and the effect of muscle activity on joint shape. (A): Brightfield lateral and ventral view of 5 dpf zebrafish expressing Tg(Col2a1aBAC:mcherry) cartilage marker MC=Meckel′s cartilage, PQ=palatoquadrate, CH=ceratohyal. (B): The number of mouth openings per minute at 58 h post-fertilisation (hpf), (n=10), 72 hpf (n=6), 96 hpf (n=6) and 120 hpf (n=6). ns= not significant, ***=Pd0.001. (C, D, E): Confocal image stacks of ventral zebrafish jaws at 3 (C), 4 (D), and 5 dpf (E), marked with a Tg(Col2a1aBAC:mcherry) cartilage marker. The medial and lateral sides of the elements are labelled M and L and the anterior and posterior surfaces of the elements are labelled A and P. IZ marks the interzone. (C′, D′, E′): 3D Avizo reconstructions from the confocal datasets: cartilage marked red, interzone marked yellow. RAP denotes the retroarticular process of the Meckel′s cartilage (F): Box and whisker plot showing the interval between the anterior MC and posterior PQ elements on the medial and lateral side of the joints at 3 dpf (n=12), 4 dpf (n=16), 5 dpf (n=13) and in 5 dpf myod (n=4) and 5 dpf anaesthetised zebrafish (n=8), ns=not significant, ***=Pd0.001. Negative measurements indicate an overlap of the anterior MC and posterior PQ elements. (G, H): confocal image stacks of the ventral jaws of 5 dpf myod mutant (G) and 5 dpf anaesthetised zebrafish (H) marked by Tg(Col2a1aBAC:mcherry) cartilage marker. (G′, H′) 3D Avizo reconstructions from the confocal datasets. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)