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Fig. S3 Hepatic steatosis in overfed zebrafish. (A) Representative H&E, and PAS staining of liver sections from controls (left) and overfed fish after 1 week of treatment (right). Both controls and treated animals were old male fish. Size bar 20 mm. (B) Quantitation of Oil red O stained area in liver sections from young males, old males, young females and old females zebrafish at baseline and after 1 week of overfeeding. The positive areas were measured with Image J (Schneider et al., 2012) and normalized on the total area of the section. The analysis has been carried out in three sections from 5 fish per subgroups. (* male young: p=0.033; ** male old: p=0.009; ° female young: p=0.283; °° female old: p=0.014; vs. respective baseline).