ZFIN Image: Bednarek et al., 2015, Fig. 3

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Figure Caption

Fig. 3

The Absence of Telomerase Severely Affects Cardiomyocyte Proliferation

(A and B) Cardiac cells positive for PCNA in UI WT and tert^-/- zebrafish hearts and in hearts at the indicated days after cryoinjury. (A) PCNA⁺ total cardiac cells. (B) PCNA⁺ cardiomyocytes. Data are means ± SEM of the percentage of PCNA⁺ cells (A) or number of PCNA⁺ cardiomyocytes per cardiac ventricle section (at least 3 sections/animal from the indicated number of animals) (B). p < 0.05 compared with WT samples (Mann-Whitney test).

(C) WT and tert^-/- heart sections immunostained with anti-MHC to mark cardiomyocytes (red) and anti-PCNA to mark cells in S phase (green). Nuclei were counterstained with DAPI (blue). The bottom rows show the location of PCNA⁺ cardiomyocytes during regeneration (yellow circles). The nuclear area is shown in magenta. Dotted lines outline the ventricle and injured area. Asterisks mark the initial injury site. Insets show high-magnification views of representative PCNA⁺ cardiomyocytes in the boxed areas. Scale bars, 100 µm (whole-heart views) and 10 µm (magnifications).

(D and E) Quantification of pH3⁺ and BrdU-labeled cardiac cells (D) and cardiomyocytes (E) at 7 and 14 dpi. Data are means ± SEM. p < 0.05, p < 0.01 (unpaired Student’s t test).