Fig. 5

Fig. 5

Tead-binding-deficient yap^ΔTB/ΔTB mutants lack RPE but maintain yap mRNA and Yap protein levels. (A) The Tead-binding-deficient yap^ΔTB/ΔTB zebrafish mutant. (B-D) 48hpf whole eyes showing that yap^-/- and yap^ΔTB/ΔTB mutants lose a subset of RPE cells. Dashed lines indicate the border of the eye. (E) qRT-PCR analysis of whole embryos at 32hpf revealing a decrease in yap (1.6-fold, *P=0.0052) and taz (1.15-fold, *P=0.0038) mRNA in yap^ΔTB/ΔTB mutants. Dotted line indicates the normalized expression levels of yap and taz in wild-type embryos. An unpaired t-test was performed and statistical significance was determined using the Holm-Sidak method. Error bars indicate s.e.m. (F-G′′′) Yap protein expression in yap^+/ΔTB (F-F′′′) and yap^ΔTB/ΔTB (G-G′′′) at 28hpf. Yap protein is present in flattened RPE nuclei (arrows) and periocular cells in yap^+/ΔTB and yap^ΔTB/ΔTB embryos.