ZFIN Image: Miesfeld et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Yap and Tead1a zebrafish protein interactions are conserved. (A) Schematic of the zebrafish Yap and Tead binding domain (BD) interaction sites. (B) Immunoprecipitation of zebrafish Yap and Tead1a wild-type and Tead-binding-deficient Yap (Yap S54A) and Yap-binding-deficient Tead1a (Tead1a Y417H) isoforms. All the mutated protein variants lose the ability to interact, in contrast to the wild-type proteins. Immunoprecipitation (IP) was with an anti-Flag antibody. (C-E′′) Whole eyes (C-D′) and sections (E-E′′) showing RPE loss surrounding the optic nerve head after overexpression of Tead1a Y417H. Photoreceptors are red owing to late expression of the rx3:Gal4 driver in these cells. Arrows indicate areas lacking RPE.

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Acknowledgments

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