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Fig. S2
Transgene expression is localized to sensory hair cells of the inner ear and lateral line - Morphological assessment.
In order to determine the identity of the cells expressing EGFP reporter gene in the inner ear and lateral line, co-labeling of EGFP (immunohistochemistry) and actin (TRITC-phalloidin) was performed on fixed embryos. Embryos from Tg(B1B2-761bp:EGFP) transgenic line were analyzed at 19hpf (A), 40hpf (B-D) and 60 hpf (E-J). For each developmental stage, EGFP (green) and actin (red) were visualized on an upright spinning disk microscope ; a merge of the two signals is also represented. Inserts show higher magnification of hair cells from the observed sensory patch: tether cells (devoid of hair bundle at 19 hpf) (A), and differentiated hair cells from the anterior maculae (B,F), the posterior maculae (C,G), anterior cristae (E), posterior cristae (H), and neuromasts (D,I,J). Images are z-projections of stacks allowing to visualize together hair cell body and bundle within one sensory patch. Neuromasts are shown entirely (D,I) or only in their most apical part (J). Scale bar = 25 µm.
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Full text @ Dev. Neurobiol.