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Fig. S5
Expression of nAg homologues in zebrafish. a-f) Whole mount mRNA ISH of agr2. c′-f′) Longitudinal sections of the rays using whole mount ISH. Agr2 mRNA is expressed in the mucous secreting cells of the entire epidermis in both non-amputated (a,b) and amputated fins (c-f). During regeneration, agr2 is also expressed in the mucous secreting cells present in the newly formed WE. There are no obvious differences between control (a,c,e) and denervated fins (b,d,f) in the amount of agr2- positive cells, in both non-amputated fins and during regeneration. g-k) Whole mount mRNA ISH of ag1. g) Ag1 mRNA is expressed in the mucous secreting cells of the larvae body at 48 hours post fertilization (hpf). h-k) Longitudinal sections of the rays using whole mount ISH show wide expression of ag1 in the epidermis and WE of adult fins, as early as 0.5 dpa, and throughout regeneration. There are no differences in ag1 expression between control and denervated fins, in both non-amputated and amputated fins.