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Fig. 3
Cell cycle analysis of transgenic zebrafish embryos expressing PCNA-CB. (A) Overview of the dorsal midbrain of a wnt1:gal4,UAS:GFP (green); UAS:PCNA-CB (magenta) double transgenic embryo at 38hpf. (B) Unaltered cell cycle progression in PCNA-CB-expressing, wnt1-positive neural progenitors. PCNA-CB signal transitions from a speckled configuration (S phase) to an evenly distributed one (G2), which precedes mitosis (M). Arrowheads indicate a cell progressing through its last cycle before terminally differentiating into two daughter neurons. Time-stamps: h:min:s. Scale bars: 50µm in A; 20µm in B.
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