ZFIN Image: Képiró et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Cellular and Subcellular Targeting of Myosin 2 in Live M2 Cells Reveal Mechanistic Details of Myosin 2 Function in Blebbing

(A) M2 cells were incubated at different concentrations of ABleb for 60 min in the dark and their blebbing indices were determined at the indicated times.

(B) 2PM bright-field images of blebbing M2 cells in the presence of 1 µM ABleb before (pre-tattooing) and after (post-tattooing) 2P irradiating one of them (indicated by red square). See also Movies S2 and S3. Scale bar represents 10 µm.

(C) Relative fluorescence of M2 cells during 2P irradiation cycles in the presence of 1 µM ABleb or NBleb.

(D) Normalized blebbing indices of M2 cells in the presence of 1 µM ABleb or 1 µM (+)-ABleb following 2P irradiation and 10 µM ABleb without irradiation.

(E) Fluorescence (lower panels) and bright-field (upper panels) images of an M2 cell before (pre-tattooing) and after 2P irradiation (post-tattooing) half of the cell (red square) in the presence of 1 µM ABleb. The diffusion of ABleb-myosin 2 covalent complexes was followed for 20 min.

(F) Normalized blebbing indices (left panel) and bleb size (right panel) of irradiated and non-irradiated sides of hemi-tattooed M2 cells pre- and 20 min post-tattooing. Data represent means ± SD (n = 4). See also Movies S4 and S5. Scale bar represents 10 µm.

Acknowledgments

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