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Fig. S5
miR-9 overexpression strategy and stabilization of dicer, miR-9 and ngn1 hindbrain expression upon dicer-3′UTR protection. Related to Figure 7.
(A) Cartoon describing the strategy adopted for the inducible miR-9 gain-of-function experiment (see Experimental Procedures for details).
(B) Fluorescent and bright-field images of whole-mount zebrafish embryos at 48 hpf injected with the Hsp70-miR-9mCherry plasmid at the one-cell stage and heatshocked at 35 hpf for 15 min at 42°C. Anterior is to the left.
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Acknowledgments
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Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell