|
Fig. S1
LPA Stimulates Stable-Bleb Cell Transformation In Vitro, Related to Figure 2
(A) Percentage of polarized stable-bleb cells cultured in suspension in the presence of 100 µM LPA for 10 min (n = 219) or different serum growth factors hEGF (n = 143), mFGF-8b+rhFGF-3 (n = 121), and PDGF-AA (n = 162) at a concentration of 100 ng/µl for 30 min.
(B) Percentage of polarized stable-bleb cells cultured in suspension at varying levels of LPA for 10 min.
(C) Bright-field and fluorescence time-lapse images of Myl12.1-eGFP (myosin II) localization during reversible polarization of progenitor cells obtained from Tg(actβ1:myl12.1eGFP) transgenic embryos. Red arrow denotes local application of 100 µM LPA by a micropipette. Blue arrow marks removal of micropipette. Yellow arrows indicate polarized stable-bleb cells.
(D) Lifeact-GFP localization in progenitor cells obtained from Tg(actβ1:lifeact-GFP) embryos and cultured in DMEM-F12 medium alone (Ctrl, top) and in the presence of 10 µM LPA (bottom). Yellow arrows mark blebs.
(E) Bright-field time-lapse images of a polarized progenitor cell cultured in suspension in the presence of 30 µM LPA. Red arrow marks application of 500 nM Jasplakinolide to the culture medium. All cells were obtained from embryos at sphere stage (4 hpf). All scale bars, 10 µm.
Reprinted from Cell, 160, Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P., Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility, 673-685, Copyright (2015) with permission from Elsevier. Full text @ Cell